Prof. Dr. Marc Nowaczyk
The role of small single transmembrane helix proteins in cyanobacteria
Ruhr University Bochum (Germany), Plant Biochemistry
Cyanobacteria are the only prokaryotes performing oxygenic photosynthesis. Multisubunit membrane protein complexes play an essential role in this process and all of them – as far as being analyzed – contain up to 10 small (<50 AA) µ-proteins with at least one single transmembrane helix (µTMH proteins). Small size paired with often extreme hydrophobicity severely hamper their discovery but we have developed a well-suited analysis pipeline by combining chromatographic purification of affinity tagged complexes with subsequent in-depth mass spectrometry analysis. This approach in combination with sequence analysis revealed novel µTMH candidates that will be targeted by reverse genetics and biochemical characterization. Moreover, previously uncharacterized cyanobacterial membrane protein complexes (e.g. Cytochrome-bd oxidase) will be isolated and analyzed for the presence of µTMH proteins. The final goal is a comprehensive view on the role of µTMH proteins in cyanobacteria.
- Wulfhorst H, Franken LE, Wessinghage T, Boekema EJ, Nowaczyk MM (2014) The 5 kDa Protein NdhP Is Essential for Stable NDH-1L Assembly in Thermosynechococcus elongatus. PLoS One 9:e103584
- Nowaczyk MM, Krause K, Mieseler M, Sczibilanski A, Ikeuchi, M, Rögner M (2012) Deletion of psbJ leads to accumulation of Psb27–Psb28 photosystem II complexes in Thermosynechococcus elongatus. BBA Bioenergetics 1817:1339-1345
- Nowaczyk MM, Wulfhorst H, Ryan CM, Souda P, Zhang H, Cramer WA, Whitelegge JP (2011) NdhP and NdhQ: two novel small subunits of the cyanobacterial NDH-1 complex. Biochemistry 50:1121-1125