Haloferax volcanii microproteins that contain two CPXCG motifs indicative for one-domain zinc finger proteins
Goethe-University Frankfurt (Germany), Institute for Molecular Biosciences
The halophilic archaeon Haloferax volcanii contains 45 small proteins with two CPXCG motifs that are probably regulatory one-domain zinc finger proteins. The biological functions of these proteins will be analyzed by quantification of the expression levels under many conditions and be the construction and phenotyping of deletion mutants. Tagged versions of selected XPXCG proteins will be produced in the respective deletion mutants. They will be affinity-purified to characterize post-translational modifications. Co-affinity purification will be used to identify binding partners of the CPXCG small proteins (proteins, DNA, or RNA). Alanine scanning mutagenesis will be used to analyze the importance of single amino acids for the function of CPXCG small proteins in vivo and in vitro. Last, but not least, the structures of two to three CPXCG small proteins will be solved. Together, the project will yield an unprecedented insight into the functions and importance of one-domain CPXCG motif zinc finger proteins in archaea.