SPP2002

News

  • New date for the Small Proteine Meeting in Kiel, which will now take place from September 19th – 21th, 2021!

Motivation

Small proteins are difficult to detect due to technical limitations but new technologies are emerging to enable their global profiling and systematic investigation of their physiological roles.

We aim to 1) globally identify small proteins in selected prokaryotes using comparative genomics, translatomics and peptidomics, 2) advance novel analytical tools to identify small proteins and their physiological roles, and 3) elucidate the functions and interaction partners of small proteins as well as underlying molecular mechanisms. Exploring the small proteome in Prokaryotes will not only deepen our understanding of gene expression regulation and protein function in general, but may also provide new innovative biotechnological applications and antimicrobial strategies. By disclosing representative functions and mechanisms for the products of this major class of genes in prokaryotic genomes we envisage to uncover novel unique prokaryotic principles in regulation of gene expression and assembly/­disassembly of large (membrane) complexes.

 

Projects

Archaea

Schmitz-Streit

  • Functional analysis of small proteins in Methanosarcina mazei focusing on those involved in nitrogen regulation or biofilm formation

 

Soppa

  • Haloferax volcanii small proteins that contain two CPXCG motifs indicative for one-domain zinc finger proteins

α-Proteobacteria

Narberhaus

  • Investigation of small arginine-rich proteins of the DUF1127 family from Agrobacterium tumefaciens

γ-Proteobacteria

Berghoff

  • Functional and regulatory interdependence of small proteins during membrane depolarization and persister cell formation in Escherichia coli

 

Yuan

  • Small regulatory proteins of the PhoQ/PhoP two-component system in enterobacteria

Cyanobacteria

Hagemann

  • The role of small proteins in metabolic regulation of cyanobacteria

 

Hess

  • The role of small proteins, peptides and dual function transcripts in cyanobacteria

 

Nowaczyk

  • The role of small single transmembrane helix proteins in cyanobacteria

Pathogenesis related

Dersch

  • Functional peptides implicated in the virulence of the enteric pathogen Yersinia pseudotuberculosis

 

Papenfort

  • Small proteins and dual RNA regulators in Vibrio cholera

 

Vogel

  • Functions of small proteins regulated during Salmonella infection

 

Sharma

  • Exploring small proteins in the food-borne pathogen Campylobacter jejuni

 

Engelmann and Fuchs

  • Identification and characterization of small proteins in Staphylococcus aureus

Membrane related

Langer

  • Identifying and characterizing µ-membrane proteins in prokaryotic terminal oxidases and proteomes

 

Koch

  • Small membrane proteins (SMPs) as organizers of the bacterial membrane

Microbiome

Clavel and Gulder

  • Ecology and functions of small SCIFF proteins in the gut microbiome

Phages

Sorek

  • Discovery and characterization of prophage-encoded peptides that direct communication between phages

Biotechnicals/ chemical methods

Schwalbe

  • NMR and biophysical characterization of structure and dynamics of small proteins and their complexes

 

Rappsilber

  • Functional and structural annotation of small proteins by advanced proteomics approaches

Z- Projects

Becher and Tholey

  • Z1: Proteomics and peptidomics for the identification and functional characterization of sORF encoded proteins

 

Sharma and Backofen

  • Z2: Riboprofiling and Bioinformatics